English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The development of clean or novel alternative energy has become a global trend that will shape the future of energy. In the present study, 3 microbial strains with different oxygen requirements, including  Clostridium acetobutylicum  ATCC 824,  Enterobacter cloacae  ATCC 13047 and  Kluyveromyces marxianus  15D, were used to construct a hydrogen production system that was composed of a mixed aerobic-facultative anaerobic-anaerobic consortium. The effects of metal ions, organic acids and carbohydrate substrates on this system were analyzed and compared using electrochemical and kinetic assays. It was then tested using small-scale experiments to evaluate its ability to convert starch in 5 L of organic wastewater into hydrogen. For the one-step biohydrogen production experiment, H1 medium (nutrient broth and potato dextrose broth) was mixed directly with GAM broth to generate H2 medium (H1 medium and GAM broth). Finally,  Clostridium acetobutylicum  ATCC 824,  Enterobacter cloacae  ATCC 13047 and  Kluyveromyces marxianus  15D of three species microbial co-culture to produce hydrogen under anaerobic conditions. For the two-step biohydrogen production experiment, the H1 medium, after cultured the microbial strains  Enterobacter cloacae  ATCC 13047 and  Kluyveromyces marxianus  15D, was centrifuged to remove the microbial cells and then mixed with GAM broth (H2 medium). Afterward, the bacterial strain  Clostridium acetobutylicum  ATCC 824 was inoculated into the H2 medium to produce hydrogen by anaerobic fermentation.    Results  The experimental results demonstrated that the optimum conditions for the small-scale fermentative hydrogen production system were at pH 7.0, 35°C, a mixed medium, including H1 medium and H2 medium with 0.50 mol/L ferrous chloride, 0.50 mol/L magnesium sulfate, 0.50 mol/L potassium chloride, 1% w/v citric acid, 5% w/v fructose and 5% w/v glucose. The overall hydrogen production efficiency in the shake flask fermentation group was 33.7 mL/h -1 .L -1 , and those the two-step and the one-step processes of the small-scale fermentative hydrogen production system were 41.2 mL/h -1 .L -1  and 35.1 mL/h -1 .L -1 , respectively.    Conclusion  Therefore, the results indicate that the hydrogen production efficiency of the two-step process is higher than that of the one-step process.

Feasibility of biohydrogen production from industrial wastes using defined microbial co-culture