English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background Sepsis is associated with substantial mortality rates. Antibiotic treatment is crucial, but global antibiotic resistance is now classified as one of the top ten global public health risks facing humanity. Ozone (O 3 ) is an inorganic molecule with no evident function in the body. We investigated the bactericide properties of ozone, using a novel system of extracorporeal ozone blood treatment. We hypothesized that ozone would decrease the concentration of viable Escherichia coli ( E. coli ) in human whole blood and that the system would be technically feasible and physiologically tolerable in a clinically relevant model of  E. coli  sepsis in swine. Methods The  E. coli  strain B09-11822, a clinical isolate from a patient with septic shock was used. The in vitro study treated  E. coli  infected human whole blood ( n  = 6) with ozone. The in vivo 3.5-h sepsis model randomized swine to  E. coli  infusion and ozone treatment ( n  = 5) or  E. coli  infusion and no ozone treatment ( n  = 5). Live  E. coli , 5 × 10 7  colony-forming units (CFU/mL) was infused in a peripheral vein. Ozone treatment was initiated with a duration of 30 min after 1.5 h. Results The single pass in vitro treatment decreased  E. coli  by 27%, mean 1941 to 1422 CFU/mL, mean of differences − 519.0 (95% CI − 955.0 to − 82.98,  P  = 0.0281). pO 2  increased (95% CI 31.35 to 48.80,  P  = 0.0007), pCO 2  decreased (95% CI − 3.203 to − 1.134,  P  = 0.0069), oxyhemoglobin increased (95% CI 1.010 to 3.669,  P  = 0.0113). Methemoglobin was not affected. In the sepsis model, 9/10 swine survived. One swine randomized to ozone treatment died from septic shock before initiation of the treatment. Circulatory, respiratory, and metabolic parameters were not affected by the ozone treatment.  E. coli  in arterial blood, in organs and in aerobic and anaerobic blood cultures did not differ. Hemoglobin, leucocytes, and methemoglobin were not affected by the treatment. Conclusions Ozone decreased the concentration of viable  E. coli  in human whole blood. The system was technically feasible and physiologically tolerable in porcine sepsis/septic shock and should be considered for further studies towards clinical applications.

Evaluation of an extracorporeal ozone-based bactericide system for the treatment of Escherichia coli sepsis