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In this study, a solid phase extraction method was successfully applied in the preconcentration and determination of trace levels of Cu(II) and Cd(II) ions and  Phanerochaete chrysosporium  (white rot fungus) modified bentonite was used as adsorbent. After the biosorption of Cu(II) and Cd(II) ions, metal concentrations in the samples were determined by atomic absorption spectrophotometry. pH, adsorbent amount, eluent type, sample volume, and flow rate, which are effective in the adsorption of metal ions, have been studied. 1 M HCl was used for desorption of these metal ions retained (recovery 95–100%). In addition, the effect of interfered ions has also been investigated. Sorption data were examined according to Langmuir and Freundlich adsorption equations. The results obtained show that the applied method has a high metal biosorption capacity, and Cu(II) and Cd(II) ions are successfully recovered. It was also successful in applying the proposed enrichment method to real water samples.  Recovery values between 92.3% and 97.3% were obtained for the studied metal ions. According to the results, the proposed method can be successfully applied to water analysis at 95% confidence interval.

The use of Phanerochaete chrysosporium for modification of bentonite for preconcentration and determination of heavy metals