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BackgroundTrimethylamine (TMA) is a marker used for monitoring the quality of seafood because it is the primary component of the “fishy” odor.MethodsThe levels of TMA in seafood samples were directly measured by helium-plasma ionization mass spectrometry (HePI-MS). Each sample was directly exposed to the HePI source, and the intensity of the m/z 60 signal for protonated TMA was monitored by a selected-ion-recording (SIR) protocol. Using a set of TMA-spiked water standards, the TMA levels in seafood samples were quantified.ResultsThe signal intensity of the m/z 60 ion from shrimp samples maintained at room temperature for 2 days can be attenuated to baseline levels by adding lime juice. The amounts of TMA in samples of salmon and shrimp recovered from some sushi preparations, and in squid samples, were found to be 0.24 μg, 0.16 μg, and 17.2 μg per gram, respectively.ConclusionsHePI-MS is an efficient technique to screen and monitor the TMA content and assess the quality of seafood.

Screening freshness of seafood by measuring trimethylamine (TMA) levels using helium-plasma ionization mass spectrometry (HePI-MS)