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The most well established tactic for the analysis of monosaccharaides, such as glucose, relies on derivatization procedures, using reagents as 2,4-dinitrophenylhydrazine (DNPH). Usually, the instability of the formed imine product deteriorates the detection of trace amounts of the sugar; rendering the spectrophotometric analysis of monosaccharaides extremely challenging.

Stability determination for cyclized 2,4-dinitrophenyl hydrazone derivative of glucose