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In this study the concept of biofilm accumulation in the sand column was promoted to assess the changes in hydraulic conductivity and concentration of organic contaminants of the synthetic leachate. Four different combinations of column study were carried out using synthetic leachate as a substrate solution. Mixed and stratified mode of experiments with two different sizes (0.3 mm and 0.6 mm) of sand grains were used for column filling. Two columns were acting as a blank, the remaining two columns amended with mixed microbial cultures which were isolated from leachate. The column was operated with continuous synthetic leachate supply for 45 days. The results indicated that the highest hydraulic conductivity reduction occurred in the mixed sand microbial column with 98.8% when compared to stratified sand microbial column. The analysis of organic contaminants of the effluent leachate was also clearly shown that the mixed sand amended with microbes poses a suitable remedial measure when compared to natural and synthetic liners for controlling the leachate migration in the subsurface environment.

Bioclogging in porous media: influence in reduction of hydraulic conductivity and organic contaminants during synthetic leachate permeation