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Background  Forward genetics approaches are not popularly applied in non-model plants due to their complex genomes, long life cycles, backward genetic studies etc. Researchers have to adopt reverse genetic methods to characterize gene functions in non-model plants individually, the efficiency of which is usually low.    Results  In this study, we report a gain-of-function in  Arabidopsis  (GAINA) strategy which can be used for batch identification of functional genes in a plant species. This strategy aims to obtain the gain-of-function of rubber tree genes through overexpressing transformation ready full-length cDNA libraries in  Arabidopsis . An initial transformation test produced about two thousand independent transgenic  Arabidopsis  lines, in which multiple obvious aberrant phenotypes were observed, suggesting the gain-of-function of rubber tree genes. The transferred genes were further isolated and identified. One gene identified to be metallothionein-like protein type 3 gene was further transferred into  Arabidopsis  and reproduced a similar aberrant phenotype.    Conclusion  The GAINA system proves to be an efficient tool for batch identification of functional genes in  Hevea brasiliensis , and also applicable in other non-model plants.    Electronic supplementary material  The online version of this article (doi:10.1186/s40064-016-3523-4) contains supplementary material, which is available to authorized users.

Gain-of-function in Arabidopsis (GAINA) for identifying functional genes in Hevea brasiliensis