English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

We performed annealing control primer (ACP)-based differential-display reverse transcription-polymerase chain reaction (DDRT-PCR) to isolate differentially expressed genes (DEGs) from the stage IV ovary and ovotestis of the rice field eel,  Monopterus albus . Using 20  arbitrary ACP primers, 14 DEG expressed-sequence tags were identified and sequenced. The transcriptional expression of one DEG,  G2 , was significantly greater in the ovotestis than the stage IV ovary. To understand the role of  G2  in sex inversion,  G2  cDNA was cloned and semi-RT-PCR, real time PCR were performed during gonad development. The full-length  G2  cDNA was 650 base pairs (bp) and it comprised a 5′-untranslated region (UTR) of 82 bp, a 3′-UTR of 121 bp and an open reading frame of 444 bp that encoded a 148-amino acid protein. The expression of  G2  was weak during early ovarian development  until the stage IV ovary, but expression increased significantly with gonad development. We speculate that  G2  may play an important function during sex inversion and testis development in the rice field eel, but the full details of the function of this gene requires further research.

Cloning and transcriptional expression of a novel gene during sex inversion of the rice field eel (Monopterus albus)