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Background  The assessment of Hospital-acquired infections due to multidrug-resistant bacteria involves the use of a variety of commercial and laboratory-developed tests to detect antimicrobial resistance genes in bacterial pathogens; however, few are evaluated for use in low- and middle-income countries.    Methods  We used whole-genome sequencing, rapid commercial molecular tests, laboratory-developed tests and routine culture testing.    Results  We identified the carriage of the metallo-β-lactamase  bla  VIM-2  and  bla  IMP-18  alleles in Carbapenem-Resistant  Pseudomonas aeruginosa  infections among children in Costa Rica.    Conclusions  The  bla  IMP-18  allele is not present in the most frequently used commercial tests; thus, it is possible that the circulation of this resistance gene may be underdiagnosed in Costa Rica.

antimicrobial resistance and infection control

Carriage of two carbapenem-resistance genes in Pseudomonas aeruginosa isolated from hospital-acquired infections in children from Costa Rica: the importance of local epidemiology