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Background   Burkholderia pseudomallei  is an emerging pathogen that causes melioidosis, a serious and potentially fatal disease which requires prolonged antibiotics to prevent relapse. However, diagnosis of melioidosis can be difficult, especially in culture-negative cases. While metabolomics represents an uprising tool for studying infectious diseases, there were no reports on its applications to  B. pseudomallei . To search for potential specific biomarkers, we compared the metabolomics profiles of culture supernatants of  B. pseudomallei  (15 strains),  B. thailandensis  (3 strains),  B. cepacia  complex (14 strains),  P. aeruginosa  (4 strains) and  E. coli  (3 strains), using ultra-high performance liquid chromatography-electrospray ionization-quadruple time-of-flight mass spectrometry (UHPLC-ESI-Q-TOF-MS). Multi- and univariate analyses were used to identify specific metabolites in  B. pseudomallei .    Results  Principal component and partial-least squares discrimination analysis readily distinguished the metabolomes between  B. pseudomallei  and other bacterial species. Using multi-variate and univariate analysis, eight metabolites with significantly higher levels in  B. pseudomallei  were identified. Three of the eight metabolites were identified by MS/MS, while five metabolites were unidentified against database matching, suggesting that they may be potentially novel compounds. One metabolite, m/z 144.048, was identified as 4-methyl-5-thiazoleethanol, a degradation product of thiamine (vitamin B 1 ), with molecular formula C 6 H 9 NOS by database searches and confirmed by MS/MS using commercially available authentic chemical standard. Two metabolites, m/z 512.282 and m/z 542.2921, were identified as tetrapeptides, Ile-His-Lys-Asp with molecular formula C 22 H 37 N 7 O 7  and Pro-Arg-Arg-Asn with molecular formula C 21 H 39 N 11 O 6 , respectively. To investigate the high levels of 4-methyl-5-thiazoleethanol in  B. pseudomallei , we compared the thiamine degradation pathways encoded in genomes of  B. pseudomallei  and  B. thailandensis . While both  B. pseudomallei  and  B. thailandensis  possess thiaminase I which catalyzes degradation of thiamine to 4-methyl-5-thiazoleethanol,  thiM , which encodes hydroxyethylthiazole kinase responsible for degradation of 4-methyl-5-thiazoleethanol, is present and expressed in  B. thailandensis  as detected by PCR/RT-PCR, but absent or not expressed in all  B. pseudomallei  strains. This suggests that the high 4-methyl-5-thiazoleethanol level in  B. pseudomallei  is likely due to the absence of hydroxyethylthiazole kinase and hence reduced downstream degradation.    Conclusion  Eight novel biomarkers, including 4-methyl-5-thiazoleethanol and two tetrapeptides, were identified in the culture supernatant of  B. pseudomallei .    Electronic supplementary material  The online version of this article (doi:10.1186/s13578-015-0018-x) contains supplementary material, which is available to authorized users.

cell and bioscience

Metabolomic profiling of Burkholderia pseudomallei using UHPLC-ESI-Q-TOF-MS reveals specific biomarkers including 4-methyl-5-thiazoleethanol and unique thiamine degradation pathway