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1,2-propanediol (1,2-PDO) is a versatile chemical used in multiple manufacturing processes. To date, some engineered and non-engineered microbes, such as  Escherichia coli, Lactobacillus buchneri,  and  Clostridium thermosaccharolyticum,  have been used to produce 1,2-PDO. In this study, we demonstrated the production of  R - and  S -1,2-PDO using engineered  Lactococcus lactis . The L- and D-lactic acid-producing  L. lactis  strains NZ9000 and AH1 were transformed with the plasmid pNZ8048-ppy harboring  pct ,  pduP , and  yahK  genes for 1,2-PDO biosynthesis, resulting in  L. lactis  LL1 and LL2, respectively. These engineered  L. lactis  produced  S - and  R -1,2-PDO at concentrations of 0.69 and 0.50 g/L with 94.4 and 78.0%  ee  optical purities, respectively, from 1% glucose after 72 h of cultivation. Both 1% mannitol and 1% gluconate were added instead of glucose to the culture of  L. lactis  LL1 to supply NADH and NADPH to the 1,2-PDO production pathway, resulting in 75% enhancement of  S -1,2-PDO production. Production of  S -1,2-PDO from 5% mannitol and 5% gluconate was demonstrated using  L. lactis  LL1 with a pH–stat approach. This resulted in  S -1,2-PDO production at a concentration of 1.88 g/L after 96 h of cultivation. To our knowledge, this is the first report on the production of  R - and  S -1,2-PDO using engineered lactic acid bacteria.   Supplementary Information  The online version contains supplementary material available at 10.1186/s13568-021-01276-8.

Production of R- and S-1,2-propanediol in engineered Lactococcus lactis