English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

The basidiomycetous yeast  Pseudozyma antarctica  (currently designated  Moesziomyces antarcticus ) produces extracellular enzymes and glycolipids, including mannosylerythritol lipids (MELs), which are biosurfactants. Strain GB-4(0) of this species was previously isolated from rice husks and produces biodegradable plastic-degrading enzyme ( Pseudozyma antarctica  esterase; PaE). In this study, we generated a MEL biosynthesis-deficient strain (∆Pa EMT1 ) by deleting the gene Pa EMT1 , which is essential to MEL biosynthesis in strain GB-4(0). The resulting ∆Pa EMT1  strain showed deficient PaE activity, and the corresponding signal was hardly detected in its culture supernatant through western blotting analysis using rabbit anti-PaE serum. On the other hand, the relative expression of the gene Pa CLE1 , encoding PaE, was identical between GB-4(0) and ∆Pa EMT1  based on quantitative real-time PCR. When strain ∆Pa EMT1  was grown in culture media supplemented with various surfactants, i.e., Tween20, BRIJ35 and TritonX-100, and MELs, PaE activity and secretion recovered. We also attempted to detect intracellular PaE using cell-free extract, but observed no signal in the soluble or insoluble fractions of ∆Pa EMT1 . This result suggested that the Pa CLE1  gene was not translated to PaE, or that expressed PaE was degraded immediately in ∆Pa EMT1 . Based on these results, MEL biosynthesis is an important contributor to PaE production.   Electronic supplementary material  The online version of this article (10.1186/s13568-019-0825-2) contains supplementary material, which is available to authorized users.

amb express

Deficiency of biodegradable plastic-degrading enzyme production in a gene-deletion mutant of phyllosphere yeast, Pseudozyma antarctica defective in mannosylerythritol lipid biosynthesis