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Calebin-A is a curcuminoid compound reported to be present in  Curcuma longa  rhizome. The current study was aimed to isolate and characterize calebin-A from  Curcuma caesia  rhizome and its production through biotransformation approach using endophytic fungus.  C. caesia  rhizomes of different ages were subjected to analysis in order to investigate the age at which maximum calebin-A content is present. HP-TLC profiles, HPLC retention times and mass spectrometry detector confirmed the occurrence of calebin-A in  C. caesia  rhizomes of 12 to 14 months of age but not in rhizomes younger to 12 months. Furthermore, an endophytic fungus strain, EPE-10 that was isolated from the medicinal plant  C. caesia  was identified as  Ovatospora brasiliensis  based on morphological and molecular characteristics. This strain  O. brasiliensis  was deposited to the culture collected centre, MTCC Chandigarh, India under the Budapest treaty and was designated with the Accession Number MTCC 25236. Biotransformation process was carried out at 37 ± 0.5 °C with shaking for 7 days after addition of 0.01% w/v curcumin. Extraction of biotransformed products was done by following partition method and the extracts obtained were analyzed using HPTLC, HPLC and LCMS. The data of the study suggested that  O. brasiliensis  MTCC 25236 was found to convert curcumin to calebin-A in a time dependant manner with optimum conversion at 48 h. Furthermore,  O. brasiliensis  MTCC 25236 was found to be positive for the Baeyer–Villiger monooxygenase (BVMOs) enzyme activity which could possibly be the mechanism of this bioconversion. The results of this study for the first time indicated that the endophytic fungus identified as  O. brasiliensis  MTCC 25236 isolated from the  C. caesia  rhizome could be a possible source for naturally producing calebin-A.

Bioconversion of curcumin into calebin-A by the endophytic fungus Ovatospora brasiliensis EPE-10 MTCC 25236 associated with Curcuma caesia