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In an aqueous buffer system, serious reverse and side reactions were found in the asymmetric reduction of 2-octanone with  Saccharomyces cerevisiae . However, some nonionic surfactants added to the aqueous buffer system improved the bioreduction process by decreasing the reverse and side reaction rates in addition to effectively increasing the average positive reaction rate. Further, a shorter carbon chain length of hydrophilic or hydrophobic moieties in surfactants resulted in a higher yield of ( S )-2-octanol. The alkylphenol ethoxylate surfactants had a less influence than polyoxyethylenesorbitan trialiphatic surfactants on the product  e.e . It suggested that the product  e.e.  resulting from the change of carbon chain length of the hydrophobic moieties varied markedly compared with the change of carbon chain length of the hydrophilic moiety. Emulsifier OP-10 and Tween 20 markedly enhanced the yield and product  e.e.  at the concentration of 0.4 mmol L −1  with a yield of 73.3 and 93.2%, and the product  e.e.  of 99.2 and 99.3%, respectively, at the reaction time of 96 h.

Nonionic surfactants and their effects on asymmetric reduction of 2-octanone with Saccharomyces cerevisiae