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Trichoderma brevicompactum  and  T. arundinaceum  both can synthesize trichodermin with strong antifungal activity and high biotechnological value. The two  Trichoderma  species have a  tri  cluster, which includes seven genes ( tri14 ,  tri12 ,  tri11 ,  tri10 ,  tri3 ,  tri4 , and  tri6 ) that encode transport and regulatory enzymes required for the biosynthesis of trichodermin. Here, we isolated  T. brevicompactum  0248 transformants with disrupted  tri11 ,  tri4 , or  tri3  gene. We also described the effect of  tri11 ,  tri3 , or  tri4  deletion on the expression of other genes in the  tri  cluster. Targeted  Δtri3  knockout mutant exhibited a sharp decline in the production of trichodermin, and trichodermol, which is a substrate for trichodermin production, accumulated. Thus, the results demonstrated that  tri3  was responsible for the biosynthesis of trichodermin, and the  tri3  gene-encoded enzyme catalyzed the acetylation reaction of the hydroxy group at C-4 of the trichodermin skeleton. In addition,  tri4  and  tri11  deletion mutants were generated to evaluate the roles of  tri4  and  tri11  in trichodermin biosynthesis, respectively. Deletion mutant strain  Δtri4  or  Δtri11  did not produce trichodermin in  T. brevicompactum , indicating that  tri4  and  tri11  are essential for trichodermin biosynthesis. This is the first to report the function of  tri3 ,  tri4  and  tri11  in  T. brevicompactum , although the role of  tri4  and  tri11  has already been described for  T. arundinaceum  by Cardoza et al. (Appl Environ Microbiol 77:4867–4877,  2011 ).   Electronic supplementary material  The online version of this article (10.1186/s13568-018-0585-4) contains supplementary material, which is available to authorized users.

Tri11, tri3, and tri4 genes are required for trichodermin biosynthesis of Trichoderma brevicompactum