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Bovine tuberculosis is a disease caused by  Mycobacterium bovis  ( M. bovis ) that leads to great economic losses in cattle production. The discovery of a reasonable bioagent to reduce  M. bovis  infection risk and environment contamination becomes significant and urgent. Previous study reported that human β-defensin-3 (HBD3) participated in  Mycobacterial  immunity and was recognized as a suitable candidate reagent. However, its minimal inhibitory concentration to  M. bovis  is not yet reported. In this study, we first purified HBD3 protein by recombinant-DNA technology and prokaryotic expression system. Subsequently, anti-bacterial tests were used to evaluate the basic bioactivity of the protein. Results revealed that recombinant HBD3 (rHBD3) protein inhibits  Staphylococcus  multiplication but not the host  Escherichia coli . The growth curve of  M. bovis  showed that rHBD3 protein controls the proliferation of  M. bovis  in 20 μg/ml concentration. In addition, rHBD3 protein-incubated  M. bovis  exhibited reduced infectivity to alveolar epithelial cells and macrophages. In conclusion, the expression of rHBD3 protein is a potential ideal bio-regent for reducing  M. bovis  infection.

Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacity