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As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a  DHN  gene named  CsLEA11  was identified and characterized from  Cucumis sativus . Sequence analysis of CsLEA11 showed that it is a Y 3 SK 2 -type DHN protein rich in hydrophilic amino acids. Expression analyses revealed that the transcription of  CsLEA11  could be significantly induced by heat and cold stress. The recombinant plasmid was transformed into  Escherichia coli  BL21 and isopropy-β- d -thiogalactoside (IPTG) was used to induce recombinant  E. coli  to express  CsLEA11  gene. Overexpression of  CsLEA11  in  E. coli  enhanced cell viability and conferred tolerance to heat and cold stress. Furthermore, CsLEA11 protein could protect the activity of lactate dehydrogenase (LDH) under heat stress. Taken together, our data demonstrate that  CsLEA11  might function in tolerance of cucumber to heat and cold stress.   Electronic supplementary material  The online version of this article (doi:10.1186/s13568-017-0483-1) contains supplementary material, which is available to authorized users.

Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli