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Ethanol is by volume the largest fermentation product. During ethanol production by  Saccharomyces cerevisiae  about 4-5% of the carbon source is lost to glycerol production. Different approaches have been proposed for improving the ethanol yield while reducing glycerol production. Here we studied the effect of reducing glycerol export/formation through deletion of the aquaglyceroporin gene  FPS1  together with expressing  gapN  encoding NADP + -dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from  Streptococcus mutans  and overexpressing the ATP-NADH kinase gene  UTR1  from  S. cerevisiae . This strategy will allow reducing the redox balance problem observed when the glycerol pathway is blocked, and hereby improve ethanol production. We found that our strategy enabled increasing the ethanol yield by 4.6% in the case of the best producing strain, compared to the reference strain, without any major effect on the specific growth rate.   Electronic supplementary material  The online version of this article (doi:10.1186/s13568-014-0086-z) contains supplementary material, which is available to authorized users.

Enhanced ethanol production and reduced glycerol formation in fps1∆ mutants of Saccharomyces cerevisiae engineered for improved redox balancing