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Flue gases from a power plant based on waste combustion were tested as a carbon dioxide (CO 2 ) source for growing  Chlamydomonas reinhardtii . To achieve recognition as an environmentally friendly hydrogen production method, waste gases should be used to grow this hydrogen-producing microalgae. The algae were grown in undiluted flue gas containing 11.4±0.2% CO 2  by volume, in diluted flue gas containing 6.7±0.1% or 2.5±0.0% CO 2 , and in pure liquid CO 2  at a concentration of 2.7±0.2%. The NO x  concentration was 45±16 mg m -3 , the SO 2  concentration was 36±19 mg m -3 , the HCl concentration 4.1±1.0 mg m -3  and the O 2  concentration 7.9±0.2% in the undiluted flue gas. Undiluted flue gas reduced the dry weight production by around 20-25% when grown at a photon flux density (PFD) of 300 μmol m -2  s -1  artificial light and at 24 or 33°C, compared with the other treatments. A less negative effect was found at the highest flue gas concentration when the algae were grown at 75 μmol m -2  s -1  PFD. Growing the algae outdoors at a day length of 12.5 h and a temperature of around 24°C, the dry weight production was higher (about 15%) in the 2.6% CO 2  flue gas treatment compared with all other treatments. Reducing the light level by 30% through shading did not affect the dry weight production. Calculated on aerial basis the productivity reached approximately 70 g m -2  day -1  in the 300 μmol m -2  s -1  PFD treatment (corresponding to 25 mol m -2  day -1 ) and approximately 17 g m -2  day -1  in the 75μmol m -2  s -1  PFD treatment (corresponding to 6.5 mol m -2  day -1 ). The outdoor production reached around 14 g m -2  day -1 . It was concluded that the negative effect of the undiluted flue gas was attributable to the high CO 2  concentration and not to the other pollutants.

The effect on growth of Chlamydomonas reinhardtii of flue gas from a power plant based on waste combustion