English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Toll-like receptor 19 (Tlr19) is a fish-specific TLR that plays a critical role in innate immunity. In the present study, we aimed to identify  tlr19  from common carp ( Cyprinus carpio  L.) and explored its expression profile, localization, adaptor, and signaling pathways. A novel  tlr19  cDNA sequence ( Cctlr19 ) was identified in common carp. Phylogenetic analysis revealed that  Cc Tlr19 was most closely related to  Danio rerio  Tlr19. Subcellular localization analysis indicates that  Cc Tlr19 was synthesized in the free ribosome and then transported to early endosomes.  Cctlr19  was constitutively expressed in all the examined tissues, with the highest expression in the brain. After poly(I:C) and  Aeromonas hydrophila  injection, the expression of  Cctlr19  was significantly upregulated in immune-related organs. In addition, the expression of  Cctlr19  was upregulated in head kidney leukocytes (HKL) upon stimulation with different ligands. Immunofluorescence and luciferase analyses indicate that  Cc Tlr19 recruited TRIF as an adaptor. Furthermore,  Cc Tlr19 can activate the expression of  ifn-1  and  viperin . Taken together, these findings lay the foundation for future research to investigate the mechanisms underlying fish  tlr19 .   Supplementary Information  The online version contains supplementary material available at 10.1186/s13567-021-00957-3.

veterinary research

Identification and functional characterization of a fish-specific tlr19 in common carp (Cyprinus carpio L.) that recruits TRIF as an adaptor and induces ifn expression during the immune response