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Background  We aimed to determine the accuracy of laser Doppler perfusion imaging (LDPI) in an animal model for hind limb ischemia.    Methods  We used a murine (C57Bl/6 mice) ischemic hind limb model in which we compared LDPI with the clinically used  99m Tc-sestamibi SPECT perfusion imaging ( n  = 7). In addition, we used the SPECT tracer  99m Tc-pyrophosphate ( 99m Tc-PyP) to image muscular damage ( n  = 6).    Results  LDPI indicated a quick and prominent decrease in perfusion immediately after ligation, subsequently recovering to 21.9 and 25.2 % 14 days later in the  99m Tc-sestamibi and  99m Tc-PyP group, respectively.  99m Tc-sestamibi SPECT scans also showed a quick decrease in perfusion. However, nearly full recovery was reached 7 days post ligation. Muscular damage, indicated by the uptake of  99m Tc-PyP, was highest at day 3 and recovered to baseline levels at day 14 post ligation. Postmortem histology supported these findings, as a significantly increased collateral diameter was found 7 and 14 days after ligation and peak macrophage infiltration and TUNEL positivity was found on day 3 after ligation.    Conclusions  Here, we indicate that LDPI strongly underestimates perfusion recovery in a hind limb model for profound ischemia.

Comparison of LDPI to SPECT perfusion imaging using 99mTc-sestamibi and 99mTc-pyrophosphate in a murine ischemic hind limb model of neovascularization