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Background  The pathogenesis and immune response to Middle East respiratory syndrome (MERS) caused by a recently discovered coronavirus, MERS-CoV, have not been fully characterized because a suitable animal model is currently not available.  18 F-Fluorodeoxyglucose ([ 18 F]-FDG)-positron emission tomography/computed tomography (PET/CT) as a longitudinal noninvasive approach can be beneficial in providing biomarkers for host immune response. [ 18 F]-FDG uptake is increased in activated immune cells in response to virus entry and can be localized by PET imaging. We used [ 18 F]-FDG-PET/CT to investigate the host response developing in nonhuman primates after MERS-CoV exposure and applied kinetic modeling to monitor the influx rate constant ( K   i  ) in responsive lymphoid tissue .Methods  Multiple [ 18 F]-FDG-PET and CT images were acquired on a PET/CT clinical scanner modified to operate in a biosafety level 4 environment prior to and up to 29 days after MERS-CoV aerosol exposure. Time activity curves of various lymphoid tissues were reconstructed to follow the [ 18 F]-FDG uptake for approximately 60 min (3,600 s). Image-derived input function was used to calculate  K   i   for lymphoid tissues by Patlak plot.    Results  Two-way repeated measures analysis of variance revealed alterations in  K   i   that was associated with the time point ( p  < 0.001) after virus exposure and the location of lymphoid tissue ( p  = 0.0004). As revealed by a statistically significant interaction ( p  < 0.0001) between these two factors, the pattern of  K   i   changes over time differed between three locations but not between subjects. A distinguished pattern of statistically significant elevation in  K   i   was observed in mediastinal lymph nodes (LNs) that correlated to  K   i   changes in axillary LNs. Changes in LNs  K   i   were concurrent with elevations of monocytes in peripheral blood.    Conclusions  [ 18 F]-FDG-PET is able to detect subtle changes in host immune response to contain a subclinical virus infection. Full quantitative analysis is the preferred approach rather than semiquantitative analysis using standardized uptake value for detection of the immune response to the virus.    Electronic supplementary material  The online version of this article (doi:10.1186/s13550-015-0143-x) contains supplementary material, which is available to authorized users.

ejnmmi research

Modeling [18F]-FDG lymphoid tissue kinetics to characterize nonhuman primate immune response to Middle East respiratory syndrome-coronavirus aerosol challenge