English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Tissue-specific stem/progenitor cells are found in various adult tissues and may have the capacity for lineage-specific differentiation, facilitating applications in autologous transplantation. Stage-specific embryonic antigen 4 (SSEA-4), an early embryonic glycolipid antigen, is expressed in cells derived from adult human pancreas exocrine tissue. Here, we examined the characteristics and lineage-specific differentiation capacity of SSEA-4 +  cells.    Methods  Human adult partial pancreas tissues were obtained from different donors and cultured in vitro. SSEA-4 +  and CA19-9 +  cells were isolated from adult human pancreas exocrine cells using magnetic-activated cell sorting, and gene expression was validated by quantitative polymerase chain reaction. To confirm in-vivo differentiation, SSEA-4 +  and CA19-9 +  cells were transplanted into the dorsal subcutaneous region of mice. Finally, morphological features of differentiated areas were confirmed by immunostaining and morphometric analysis.    Results  SSEA-4-expressing cells were detected in isolated pancreas exocrine cells from adult humans. These SSEA-4 +  cells exhibited coexpression of CA19-9, a marker of pancreatic duct cells, but not amylase expression, as shown by immunostaining and flow cytometry. SSEA-4 +  cells exhibited higher relative expression of  Oct4 ,  Nanog ,  Klf4 ,  Sox2 , and  c-Myc  mRNAs than CA19-9 +  cells. Pancreatic intralobular ducts (PIDs) were generated from SSEA-4 +  or CA19-9 +  cells in vivo at 5 weeks after transplantation. However, newly formed PIDs from CA19-9 +  cells were less abundant and showed an incomplete PID morphology. In contrast, newly formed PIDs from SSEA-4 +  cells were abundant in the transplanted area and showed a crowded morphology, typical of PIDs. Sox9 and Ngn3, key transcription factors associated with pancreatic development and regeneration, were expressed in PIDs from SSEA-4 +  cells.    Conclusions  SSEA-4-expressing cells in the adult human pancreas may have the potential for regeneration of the pancreas and may be used as a source of stem/progenitor cells for pancreatic cell lineage-specific differentiation.    Electronic supplementary material  The online version of this article (doi:10.1186/s13287-016-0422-0) contains supplementary material, which is available to authorized users.

stem cell research and therapy

Adult human pancreas-derived cells expressing stage-specific embryonic antigen 4 differentiate into Sox9-expressing and Ngn3-expressing pancreatic ducts in vivo