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Objective  Healthcare-associated infections due to Gram-negative bacteria (GNB) are a major cause of mortality and morbidity throughout the world. The purpose of the research described here was to evaluate the possibility of using an extremely low-concentration gaseous chlorine dioxide (ClO 2 , 0.01 ppmv, 0.028 mg/m 3 ) as a technique to reduce the risk of environmental infection by GNB. In this study we set up an exposure chamber (1 m 3 ) and used three types of GNB, namely  Escherichia coli ,  Pseudomonas aeruginosa  and  Acinetobacter baumannii .    Results  The extremely low-concentration gaseous ClO 2  inactivated  E. coli  (> 2 log 10  reductions, within 2 h),  P. aeruginosa  (> 4 log 10  reductions, within 2 h) and  A .  baumannii  (> 2 log 10  reductions, within 3 h) in wet conditions on glass dishes. Treatment of moist environments with extremely low-concentration gaseous ClO 2  may help to reduce the risk of environmental infection by GNB without harmful effects.

bmc research notes

Effect of extremely low-concentration gaseous chlorine dioxide against surface Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii in wet conditions on glass dishes