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Objective  Rapid, reliable, and affordable detection of  Brucella  species via the molecular methods remains a challenge. In recent years, loop-mediated isothermal amplification (LAMP) is a functional nucleic acid amplification technique offering a substitute to polymerase chain reaction (PCR). So, we compared the LAMP assay with the conventional PCR for the identification of common  Brucella  species in Iran. In this study, LAMP assay was comprehensively evaluated against the common PCR method. A group of specific LAMP primers were used to amplify a highly specific fragment from the sequence of the  Brucella abortus, bcsp31  gene. Sensitivity and specificity values of tests were done with a set of 78 (50  Brucella  and 28 non- Brucella ) strains.    Results  A dilution series of  B. abortus  DNA indicated that the LAMP reaction could reliably detect 10 (fg/µl) DNA target copies per reaction within 36 min, which is 10 times greater than the PCR assay. In summary, we conclude that LAMP assay provide accurate and fast test results to identify of common  Brucella  species in low-complexity labs, mainly in low and lower middle income countries.

Comparison of loop-mediated isothermal amplification and conventional PCR tests for diagnosis of common Brucella species