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Objectives  The silkworm  Bombyx mori (B. mori)  is an important domesticated lepidopteran model for basic and applied research. They produce silk fibres that have great economic value. The gut microbiome plays an important role in the growth of organisms. Spermidine (Spd) is shown to be important for the growth of all living cells. The effect of spermidine feeding on the gut microbiome of 5th instar  B. mori  larvae was checked. The  B. mori  gut samples from control and spermidine fed larvae were subjected to next-generation sequencing analysis to unravel changes in the bacterial community upon spermidine supplementation.    Data description  The changes in gut bacteriota after spermidine feeding is not studied before.  B. mori  larvae were divided into two groups of 50 worms each and were fed with normal mulberry leaves and mulberry leaves fortified with 50 µM spermidine. The gut tissues were isolated aseptically and total genomic DNA was extracted, 16S rRNA region amplified and sequenced using Illumina platform. The spermidine fed gut samples were shown to have abundance and diversity of the phyla  Proteobacteria, Firmicutes, Bacteroidetes,  and  Actinobacteria.

16S rRNA sequence data of Bombyx mori gut bacteriome after spermidine supplementation