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Objective  Aphids harbor a nutritional obligate endosymbiont in specialized cells called bacteriocytes, which aggregate to form an organ known as the bacteriome. Aphid bacteriomes display distinct gene expression profiles that facilitate the symbiotic relationship. Currently, the mechanisms that regulate these patterns of gene expression are unknown. Recently using computational pipelines, we identified miRNAs that are conserved in expression in the bacteriomes of two aphid species and proposed that they function as important regulators of bacteriocyte gene expression. Here using a dual luciferase assay in mouse NIH/3T3 cell culture, we aimed to experimentally validate the computationally predicted interaction between  Myzus persicae  miR-92a and the predicted target region of  M. persicae  bacteriocyte-specific secreted protein 1 (SP1) mRNA.    Results  In the dual luciferase assay, miR-92a interacted with the  SP1  target region resulting in a significant downregulation of the luciferase signal. Our results demonstrate that miR-92a interacts with  SP1  to alter expression in a heterologous expression system, thereby supporting our earlier assertion that miRNAs are regulators of the aphid/ Buchnera  symbiotic interaction.

microRNA-92a regulates the expression of aphid bacteriocyte-specific secreted protein 1