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Objective  GA A NTRY (Gene Assembly in  Agrobacterium  by Nucleic acid Transfer using Recombinase technologY) is a flexible and effective system for stably stacking multiple genes within an  Agrobacterium  virulence plasmid Transfer-DNA (T-DNA). We examined the ability of the GA A NTRY  Agrobacterium rhizogenes  ArPORT1 ‘10-stack’ strain to generate transgenic potato plants.    Results  The 28.5 kilobase 10-stack T-DNA, was introduced into Lenape potato plants with a 32% transformation efficiency. Molecular and phenotypic characterization confirmed that six of the seven tested independent transgenic lines carried the entire desired construct, demonstrating that the GA A NTRY 10-stack strain can be used can be used in a tissue culture-based callus transformation method to efficiently generate transgenic potato plants. Analysis using droplet digital PCR showed that most of the characterized events carry one or two copies of the 10-stack transgenes and that ‘backbone’ DNA from outside of the T-DNA was absent in the transgenic plants. These results demonstrate that the GA A NTRY system efficiently generates high quality transgenic potato plants with a large construct of stacked transgenes.    Electronic supplementary material  The online version of this article (10.1186/s13104-019-4493-8) contains supplementary material, which is available to authorized users.

Transgene stacking in potato using the GAANTRY system