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Objective  Bacterial canker is a destructive disease of kiwifruit caused by the Gram-negative bacterium  Pseudomonas syringae  pv.  actinidiae  ( Psa ). To understand the disease-causing mechanism of  Psa , a kiwifruit yeast two-hybrid cDNA library was constructed to identify putative host targets of the  Psa  Type Three Secreted Effector AvrPto5.    Results  In this study, we used the Mate & Plate™ yeast two-hybrid library method for constructing a kiwifruit cDNA library from messenger RNA of young leaves. The constructed library consisted of 2.15 × 10 6  independent clones with an average insert size of 1.52 kb. The screening of the kiwifruit yeast two-hybrid cDNA library with  Psa  AvrPto5 revealed the interaction of a V-type proton ATPase subunit-H, a proline rich-protein and heavy metal-associated isoprenylated plant protein 26. Among these, heavy metal-associated isoprenylated plant protein 26 showed a positive interaction with  Psa  AvrPto5 as both prey and bait.    Electronic supplementary material  The online version of this article (10.1186/s13104-019-4102-x) contains supplementary material, which is available to authorized users.

Construction of a kiwifruit yeast two-hybrid cDNA library to identify host targets of the Pseudomonas syringae pv. actinidiae effector AvrPto5