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Objective  Forkhead box P3 (FOXP3) is a master transcriptional factor of regulatory T-cells (Tregs). Recent studies have shown that FOXP3 is associated with growth inhibition of cancer cells. However, the role of FOXP3 in acute T-lymphoblastic leukemia (T-ALL) cells is not known. It was also reported that NOTCH signaling promoted the expression of FOXP3 in Tregs. However, the effect of FOXP3 on NOTCH expression in T-ALL cells is little known. Therefore, we examined the effect of  FOXP3  knockdown on the proliferation of T-ALL cells and NOTCH1 signaling.    Results  Two T-ALL cell lines Jurkat and KOPT-K1, harboring activating  NOTCH1  mutations, were transfected with small interfering RNA against  FOXP3 . Cell growth was assessed with a colorimetric assay and morphology was observed under a microscope.  FOXP3  knockdown significantly reduced cell growth and induced morphological changes suggesting apoptosis. Quantitative polymerase chain reaction revealed that  FOXP3  knockdown caused the downregulation of mRNA expression of  NOTCH1  and  HES1 . These findings suggest that FOXP3 supports the growth of T-ALL cells although this can not be generalized because we examined only two cell lines. The observed growth suppression can be partly due to the downregulation of NOTCH1 signaling. FOXP3 may be a potential therapeutic target in T-ALL.

FOXP3 knockdown inhibits the proliferation and reduces NOTCH1 expression of T cell acute lymphoblastic leukemia cells