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Objective  This study describes a restriction fragment polymorphism protocol for rapidly screening the polymorphism  SLCO1B1 c.1929A > C  in genomic DNA samples. The polymorphism  SLCO1B1 c.1929A > C  has been associated with increased activity resulting in increased hepatic uptake of drugs. Currently  SLCO1B1 c.1929A > C  is genotyped using direct sequencing techniques and 5′ nuclease based assays which can be cost prohibiting in resource limited settings. The aim of this study therefore was to design and validate a cost effective RFLP for genotyping the  SLCO1B1 c.1929A > C  polymorphism. This study was designed to investigate the effect of the polymorphism  SLCO1B1 c.1929A > C  on interindividual variability in rosuvastatin pharmacokinetics in healthy volunteers of African descent.    Results  We describe a restriction fragment length polymorphism method to genotype  SLCO1B1 c.1929A > C  polymorphism using the restriction enzyme  Ase1 . A student’s  t  test with Welch correction was used to establish association between the  SLCO1B1 c.1929A > C  variant and rosuvastatin exposure. The frequency of the  SLCO1B1 c.1929C  allele amongst Zimbabweans was 6%. The  SLCO1B1 c.1929C  allele was associated with a 75% reduction (P < 0.001) in rosuvastatin exposure when compared to individuals carrying the wild type  SLCO1B1 c.1929A  allele. Polymorphism  c.1929A > C  may therefore play a significant role in rosuvastatin response. The RFLP method is quick and cost effective.

bmc research notes

A cost effective RFLP method to genotype Solute carrier organic anion 1B1 (SLCO1B1) c.1929A&gt;C (p.Leu643Phe, rs34671512); a variant with potential effect on rosuvastatin pharmacokinetics