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Objectives  The aetiology of several human diarrhoeas has been increasingly associated with the presence of virulence factors rather than with the bacterial species hosting the virulence genes, exemplified by the sporadic emergence of new bacterial hosts. Two important virulence factors are the Shiga toxin (Stx) and the  E. coli  outer membrane protein (Eae) or intimin, encoded by the  stx  and  eae  genes, respectively. Although several polymerase chain reaction (PCR) protocols target these virulence genes, few aim at detecting all variants or have an internal amplification control (IAC) included in a multiplex assay. The objective of this work was to develop a simple multiplex PCR assay in order to detect all  stx  and  eae  variants, as well as to detect bacteria belonging to the Enterobacteriaceae, also used as an IAC.    Results  The  wecA  gene coding for the production of the Enterobacterial Common Antigen was used to develop an Enterobacteriaceae specific qPCR. Universal primers for the detection of  stx  and  eae  were developed and linked to a wecA primer pair in a robust triplex PCR. In addition, subtyping of the  stx  genes was achieved by subjecting the PCR products to restriction digestion and semi-nested duplex PCR, providing a simple screening assay for human diarrhoea diagnostic.    Electronic supplementary material  The online version of this article (10.1186/s13104-018-3457-8) contains supplementary material, which is available to authorized users.

Multiplex PCR for the simultaneous detection of the Enterobacterial gene wecA, the Shiga Toxin genes (stx1 and stx2) and the Intimin gene (eae)