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Objective  Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV infection represents a challenge. Currently there is no vaccine against HDV. Recombinant production of HDV antigen (HDAg) is the first step towards a potential vaccine candidate and the development of assays for HDV detection.    Results  This study demonstrates the expression of one HDAg isoform, S-HDAg, in  Pichia pastoris . A recombinant vector carrying a tagged gene encoding S-HDAg under the control of the methanol-inducible promoter  AOX1  was designed and integrated into  P. pastoris  X33. The protein, which was purified using a Ni 2+  affinity column and eluted at 100–150 mM imidazole, has potential as a recombinant antigen for further study.

Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris