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Background  The chloroplastic  trn L intron and the nuclear internal transcribed spacer (ITS) region were sequenced for 11  Nepenthes  species recorded in Peninsular Malaysia to examine their phylogenetic relationship and to evaluate the usage of  trn L intron and ITS sequences for phylogenetic reconstruction of this genus.    Results  Phylogeny reconstruction was carried out using neighbor-joining, maximum parsimony and Bayesian analyses. All the trees revealed two major clusters, a lowland group consisting of  N. ampullaria ,  N. mirabilis ,  N. gracilis  and  N. rafflesiana , and another containing both intermediately distributed species ( N. albomarginata  and  N. benstonei ) and four highland species ( N. sanguinea ,  N. macfarlanei ,  N. ramispina  and  N. alba ).    Conclusions  The  trn L intron and ITS sequences proved to provide phylogenetic informative characters for deriving a phylogeny of  Nepenthes  species in Peninsular Malaysia. To our knowledge, this is the first molecular phylogenetic study of  Nepenthes  species occurring along an altitudinal gradient in Peninsular Malaysia.

Phylogenetic inferences of Nepenthes species in Peninsular Malaysia revealed by chloroplast (trnL intron) and nuclear (ITS) DNA sequences