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Background  Anti-Müllerian hormone ( amh ) or Müllerian-inhibiting substance ( mis ) is a member of the transforming growth factor-β family of hormones. This gene plays a key role in vertebrate male sex-determination by inhibiting the development of the Müllerian ducts, and has been shown to be the master sex-determinant in the Patagonian pejerrey.    Results  In the lingcod,  Ophiodon elongatus , both males and females share one copy of  amh , however we have identified a second duplicate copy that appears solely in the male individuals. We have developed a PCR-based assay targeting the TGF-β domain of  amh  that provides a simple method with which to sex lingcod from a small amount of tissue. An analysis across 57 individuals gave a 100 % success rate in identifying the phenotypic sex.    Conclusions  We present a simple method to sex lingcod through non-lethal tissue sampling. A third, independent, male-specific duplication of  amh  in a teleost fish has been identified in the lingcod.    Electronic supplementary material  The online version of this article (doi:10.1186/s13104-016-2030-6) contains supplementary material, which is available to authorized users.

A PCR assay detects a male-specific duplicated copy of Anti-Müllerian hormone (amh) in the lingcod (Ophiodon elongatus)