English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   Urochloa humidicola  is a warm-season grass commonly used as forage in the tropics and is recognized for its tolerance to seasonal flooding. This grass is an important forage species for the Cerrado and Amazon regions of Brazil.  U. humidicola  is a polyploid species with variable ploidy (6X–9X) and facultative apomixis with high phenotypic plasticity. However, this apomixis and ploidy, as well as the limited knowledge of the genetic basis of the germplasm collection, have constrained genetic breeding activities, yet microsatellite markers may enable a better understanding of the species’ genetic composition. This study aimed to develop and characterize new polymorphic microsatellite molecular markers in  U. humidicola  and to evaluate their transferability to other  Urochloa  species.    Findings  A set of microsatellite markers for  U. humidicola  was identified from two new enriched genomic DNA libraries: the first library was constructed from a single sexual genotype and the second from a pool of eight apomictic genotypes selected on the basis of previous results. Of the 114 loci developed, 72 primer pairs presented a good amplification product, and 64 were polymorphic among the 34 genotypes tested. The number of bands per simple sequence repeat (SSR) locus ranged from 1 to 29, with a mean of 9.6 bands per locus. The mean polymorphism information content (PIC) of all loci was 0.77, and the mean discrimination power (DP) was 0.87. STRUCTURE analysis revealed differences among  U. humidicola  accessions, hybrids, and other  Urochloa  accessions. The transferability of these microsatellites was evaluated in four species of the genus,  U. brizantha ,  U. decumbens, U. ruziziensis,  and  U. dictyoneura , and the percentage of transferability ranged from 58.33% to 69.44% depending on the species.    Conclusions  This work reports new polymorphic microsatellite markers for  U. humidicola  that can be used for breeding programs of this and other  Urochloa  species, including genetic linkage mapping, quantitative trait loci identification, and marker-assisted selection.

Microsatellite markers for Urochloa humidicola (Poaceae) and their transferability to other Urochloa species