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Background   Fucus vesiculosus  is a brown seaweed dominant on temperate rocky shores of the northern hemisphere and, is typically distributed in the mid-upper intertidal zone. It is an external fertilizer that reproduces sexually, providing an excellent model to address conflicting theories related to mating systems and sexual selection. Microsatellite markers have been reported for several  Fucus  species, however the genomic libraries from where these markers have been isolated, have originated from two or more species pooled together ( F. vesiculosus  and  F. serratus  in one library;  F. vesiculosus ,  F. serratus  and  Ascophyllum nodosum  in a second library), or when the genomic DNA originated from only one species it was from  Fucus spiralis . Although these markers cross-amplify  F. vesiculosus  individuals, the level of polymorphism has been low for relatedness studies.    Findings  The microsatellite markers described here were obtained from an enriched genomic library, followed by 454 pyrosequencing. A total of 9 microsatellite markers were tested across 44 individuals from the North of Portugal. The mean number of alleles across loci was 8.7 and the gene diversity 0.67.    Conclusions  The high variability displayed by these microsatellite loci should be useful for paternity analysis, assessing variance of reproductive success and in estimations of genetic variation within and between populations.

Polymorphic microsatellite markers in the brown seaweed Fucus vesiculosus