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Background  Aerobic adaptations following interval training are supposed to be mediated by increased local blood supply. However, knowledge is scarce on the detailed relationship between exercise duration and local post-exercise blood supply and oxygen availability. This study aimed to examine the effect of five different exercise durations, ranging from 30 to 240 s, on post-exercise muscle oxygenation and relative changes in hemoglobin concentration.    Methods  Healthy male subjects ( N  = 18) performed an experimental protocol of five exercise bouts (30, 60, 90, 120, and 240 s) at 80 % of peak oxygen uptake    \documentclass[12pt]{minimal} 				\usepackage{amsmath} 				\usepackage{wasysym}  				\usepackage{amsfonts}  				\usepackage{amssymb}  				\usepackage{amsbsy} 				\usepackage{mathrsfs} 				\usepackage{upgreek} 				\setlength{\oddsidemargin}{-69pt} 				\begin{document}$$ \left({\overset{.}{\mathrm{V}}\mathrm{O}}_{2\mathrm{peak}}\right) $$\end{document}V  .   O    2  peakin a randomized order, separated by 5-min recovery periods. To examine the influence of aerobic fitness, we compared subjects with gas exchange thresholds (GET) above 60 %    \documentclass[12pt]{minimal} 				\usepackage{amsmath} 				\usepackage{wasysym}  				\usepackage{amsfonts}  				\usepackage{amssymb}  				\usepackage{amsbsy} 				\usepackage{mathrsfs} 				\usepackage{upgreek} 				\setlength{\oddsidemargin}{-69pt} 				\begin{document}$$ \overset{.}{\mathrm{V}}{\mathrm{O}}_{2\mathrm{peak}} $$\end{document}    V  .    O   2  peak(GET60+) with subjects reaching GET below 60 %    \documentclass[12pt]{minimal} 				\usepackage{amsmath} 				\usepackage{wasysym}  				\usepackage{amsfonts}  				\usepackage{amssymb}  				\usepackage{amsbsy} 				\usepackage{mathrsfs} 				\usepackage{upgreek} 				\setlength{\oddsidemargin}{-69pt} 				\begin{document}$$ \overset{.}{\mathrm{V}}{\mathrm{O}}_{2\mathrm{peak}} $$\end{document}    V  .    O   2  peak(GET60−).    \documentclass[12pt]{minimal} 				\usepackage{amsmath} 				\usepackage{wasysym}  				\usepackage{amsfonts}  				\usepackage{amssymb}  				\usepackage{amsbsy} 				\usepackage{mathrsfs} 				\usepackage{upgreek} 				\setlength{\oddsidemargin}{-69pt} 				\begin{document}$$ \overset{.}{\mathrm{V}}{\mathrm{O}}_2 $$\end{document}    V  .    O  2and relative changes in concentrations of oxygenated hemoglobin, deoxygenated hemoglobin, and total hemoglobin were continuously measured with near-infrared spectroscopy of the vastus lateralis muscle.    Results  Post-exercise oxygen availability and local blood supply increased significantly until the 90-s exercise duration and reached a plateau thereafter. Considering aerobic fitness, the GET60+ group reached maximum post-exercise oxygen availability earlier (60 s) than the GET60− group (90 s).    Conclusions  Our results suggest that (1) 90 s has evolved as the minimum interval duration to enhance local oxygen availability and blood supply following cycling exercise at 80 %    \documentclass[12pt]{minimal} 				\usepackage{amsmath} 				\usepackage{wasysym}  				\usepackage{amsfonts}  				\usepackage{amssymb}  				\usepackage{amsbsy} 				\usepackage{mathrsfs} 				\usepackage{upgreek} 				\setlength{\oddsidemargin}{-69pt} 				\begin{document}$$ \overset{.}{\mathrm{V}}{\mathrm{O}}_{2\mathrm{peak}} $$\end{document}    V  .    O   2  peak; whereas (2) 60 s is sufficient to trigger the same effects in subjects with GET60 + .

Relationship of post-exercise muscle oxygenation and duration of cycling exercise