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Background    Campylobacter  is a well-known bacterial pathogen for triggering acute gastroenteritis in humans both in developed and developing countries. This organism is highly resistant to fluoroquinolones. Macrolides are very much useful for the treatment of campylobacteriosis when clinical therapy is necessary. However, increasing resistance to azithromycin, a potent macrolide has been reported in  Campylobacter  in recent years. Macrolide resistance in  Campylobacter  is found mainly due to point mutation in V region of 23S rRNA.    Results  We have developed a PCR based assay, which can detect the azithromycin resistant and sensitive  Campylobacter  strains utilizing mutation responsible for the phenotype. This PCR was validated using 359  Campylobacter  strains isolated from diarrhoeal patients at Kolkata, India. Antimicrobial resistance through disk diffusion method was also performed on these strains as a gold standard. Studies through sequencing analysis further confirmed the PCR result.    Conclusion  This study describes a simple and rapid method for detection of mutation conferring macrolide resistance with additional feature of identification of sensitive strains.

gut pathogens

Development and evaluation of a PCR assay for rapid detection of azithromycin resistant Campylobacter isolated from diarrhoeal patients in Kolkata, India