English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background  Six  Sarcocystis  species are known to use cattle ( Bos taurus ) as the intermediate host, two of which,  S .  hominis  and  S .  heydorni , are zoonotic. There is a need for a method that will enable rapid identification of the  Sarcocystis  species in cattle.    Methods  The diaphragm muscles of 102 cattle from Lithuania were examined for the presence of  Sarcocystis  spp., using two different methods for species identification. Individual sarcocysts were isolated from squash preparations of the diaphragm muscle under the light microscope, followed by genetic characterisation of excised cysts using sequence analysis of the 18S rRNA ( 18S  rRNA) and cytochrome c oxidase subunit I ( cox 1) genes. The same cattle muscle samples were digested and species-specific PCR analyses targeting  cox 1 were developed to identify the  Sarcocystis  isolates to the species level.    Results  Under the light microscope, sarcocysts were detected in 87.3% of animals, and  Sarcocystis  infection was verified in all digested samples. Three species, namely  S .  cruzi  ( n  = 20),  S .  bovifelis  ( n  = 23) and  S .  hirsuta  ( n  = 6), were identified by DNA sequence analysis of isolated sarcocysts. Based on sequence analysis of  cox 1, the level of genetic variability depended on  Sarcocystis  species and geographical location. Four  Sarcocystis  species,  S .  cruzi  (96.1%),  S .  bovifelis  (71.6%),  S .  hirsuta  (30.4%) and  S .  hominis  (13.7%), were confirmed in the digested samples. In individual samples, the most common finding was two species of  Sarcocystis  (44.1%), followed by three species (26.5%), a single species (24.5%) and four species (4.9%).    Conclusions  Although examination of tissue preparations under the light microscrope did not detect any sarcocysts belonging to  S .  hominis , this species was identified in the digested samples subjected to a  cox 1-specific PCR analysis. These results demonstrate the need for effective molecular diagnosis techniques to detect  Sarcocystis  spp., which may be present at a lower prevalence and not detectable among the limited number of sarcocysts identified individually under the light microscope.

parasites and vectors

Molecular identification of four Sarcocystis species in cattle from Lithuania, including S. hominis, and development of a rapid molecular detection method