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Background  Fascioliasis caused by the trematodes  Fasciola hepatica  and  F. gigantica , is a global neglected zoonotic disease estimated to cost the livestock industry over €2.5 billion annually. Farm management measures and sustainable use of anthelmintics can, in principle, effectively control trematode infection in livestock and reduce the rate of developing anthelmintic resistance. Previously, we designed an environmental DNA (eDNA) assay to identify a common trematode intermediate host, the freshwater snail  Galba truncatula , in water sources to measure specific trematode infection risk areas on pasture-land. To improve this procedure, we now report a loop-mediated isothermal amplification (LAMP) assay to identify  G. truncatula  eDNA.    Methods  A LAMP assay was designed and optimised (e.g. temperature, time duration and primer concentration) to identify  G. truncatula  DNA. The ability of the LAMP assay to target  G. truncatula  DNA was identified, and LAMP assay limit of detection was investigated in comparison to conventional PCR. In the field, 48 water samples were collected from stream, ditch and water pool habitats in four locations at two Aberystwyth University farms over a seven week period to investigate the applicability of the LAMP assay for use on eDNA samples, in comparison to conventional PCR.    Results  The LAMP assay delivered detectable results in 30 min at 63 °C. The assay discriminated between  G. truncatula  DNA and non-target DNA, presenting a level of DNA detection comparable to conventional PCR. No significant difference was found between the ability of the LAMP and PCR assay to identify  G. truncatula  eDNA in water samples. Kappa coefficient analysis revealed a moderate level of agreement between LAMP and PCR assays.    Conclusions  This study demonstrated that the LAMP assay can detect  G. truncatula  eDNA in a simple and rapid manner. The LAMP assay may become a valuable tool to determine optimum pasture management for trematode parasite control.

Rapid detection of Galba truncatula in water sources on pasture-land using loop-mediated isothermal amplification for control of trematode infections