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Background  Malaria vector mosquitoes acquire midgut microbiota primarily from their habitat. The homeostasis of these microbial communities plays an essential role in the mosquito longevity, the most essential factor in the mosquito vectorial capacity. Our recent study revealed that silencing genes involved in regulation of the midgut homeostasis including  FN3D1 ,  FN3D3  and  GPRGr9  reduced the survival of female adult  Anopheles arabiensis  mosquitoes. In the present study, we investigate the stability of the gene silencing efficiency of mosquitoes reared in three different breeding conditions representing distinct larval habitat types: town brick pits in Jimma, flood pools in the rural land of Asendabo and roadside pools in Wolkite.    Methods  First-instar larvae of  An. arabiensis  mosquitoes were reared separately using water collected from the three breeding sites. The resulting adult females were micro-injected with dsRNA targeting the  FN3D1  gene (AARA003032) and their survival was monitored. Control mosquitoes were injected with dsRNA  Lacz . In addition, the load of midgut microbiota of these mosquitoes was determined using flow cytometry.    Results  Survival of naïve adult female mosquitoes differed between the three sites. Mosquitoes reared using water collected from brick pits and flood pools survived longer than mosquitoes reared using water collected from roadside. However, the  FN3D1  gene silencing effect on survival did not differ between the three sites.    Conclusions  The present study revealed that the efficacy of  FN3D1  gene silencing is not affected by variation in the larval habitat. Thus, silencing this gene has potential for application throughout sub-Saharan Africa.

parasites and vectors

Stability of the effect of silencing fibronectin type III domain-protein 1 (FN3D1) gene on Anopheles arabiensis reared under different breeding site conditions