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Background  Mice are susceptible to infections with the rodent filarial nematode  Litomosoides sigmodontis  and develop immune responses that resemble those of human filarial infections. Thus, the  L. sigmodontis  model is used to study filarial immunomodulation, protective immune responses against filariae and to screen drug candidates for human filarial diseases. While previous studies showed that type 2 immune responses are protective against  L. sigmodontis , the present study directly compared the impact of eosinophils, IL-5, and the IL-4R on the outcome of  L. sigmodontis  infection.    Methods  Susceptible wildtype (WT) BALB/c mice, BALB/c mice lacking eosinophils (dblGATA mice), IL-5 −/−  mice, IL-4R −/−  mice and IL-4R −/− /IL-5 −/−  mice were infected with  L. sigmodontis . Analyses were performed during the peak of microfilaremia in WT animals (71 dpi) as well as after IL-4R −/− /IL-5 −/−  mice showed a decline in microfilaremia (119 dpi) and included adult worm counts, peripheral blood microfilariae levels, cytokine production from thoracic cavity lavage, the site of adult worm residence, and quantification of major immune cell types within the thoracic cavity and spleen.    Results  Our study reveals that thoracic cavity eosinophil numbers correlated negatively with the adult worm burden, whereas correlations of alternatively activated macrophage (AAM) numbers with the adult worm burden (positive correlation) were likely attributed to the accompanied changes in eosinophil numbers. IL-4R −/− /IL-5 −/−  mice exhibited an enhanced embryogenesis achieving the highest microfilaremia with all animals becoming microfilariae positive and had an increased adult worm burden combined with a prolonged adult worm survival.    Conclusions  These data indicate that mice deficient for IL-4R −/− /IL-5 −/−  have the highest susceptibility for  L. sigmodontis  infection, which resulted in an earlier onset of microfilaremia, development of microfilaremia in all animals with highest microfilariae loads, and an extended adult worm survival.    Electronic supplementary material  The online version of this article (10.1186/s13071-019-3502-z) contains supplementary material, which is available to authorized users.

Susceptibility to L. sigmodontis infection is highest in animals lacking IL-4R/IL-5 compared to single knockouts of IL-4R, IL-5 or eosinophils