English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   Plasmodium  merozoite surface protein-1 (MSP-1) is released into the bloodstream during merozoite invasion, and thus represents a crucial malarial vaccine target. Although substantial research effort has been devoted to uncovering the genetic diversity of MSP-1 for  P. falciparum  and  P. vivax , there is minimal information available regarding the genetic profiles and structure of  P. ovale . Therefore, the aim of the present study was to determine the extent of genetic variation among two subspecies of  P. ovale  by characterizing the MSP-1 N-terminal sequence at the nucleotide and protein levels.    Methods  N-terminal of MSP-1 gene were amplified from 126 clinical samples collected from imported cases of malaria in migrant workers returning to Jiangsu Province from Africa using a conventional polymerase chain reaction (PCR) assay. The PCR products were then sequenced and analyzed using the GeneDoc, MegAlign, MEGA7 and DnaSP v.6 programs.    Results  The average pairwise nucleotide diversities ( π ) of  P. ovale curtisi  and  P. ovale wallikeri  MSP-1 genes ( pomsp1 ) were 0.01043 and 0.01974, respectively, and the haplotype diversity ( Hd ) were 0.746 and 0.598, respectively. Most of the nucleotide substitutions detected were non-synonymous, indicating that the genetic variations of  pomsp1  were maintained by positive diversifying selection, thereby suggesting their role as a potential target of a protective immune response. Amino acid substitutions of  P. ovale curtisi  and  P. ovale wallikeri  MSP-1 could be categorized into five and three unique amino acid variants, respectively.    Conclusions  Low mutational diversity was observed in  pomsp1  from the Jiangsu Province imported malaria cases; further studies will be developed such as immunogenicity and functional analysis.    Electronic supplementary material  The online version of this article (10.1186/s13071-018-3174-0) contains supplementary material, which is available to authorized users.

parasites and vectors

Limited genetic diversity of N-terminal of merozoite surface protein-1 (MSP-1) in Plasmodium ovale curtisi and P. ovale wallikeri imported from Africa to China