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Background   Haemaphysalis longicornis  is a tick of importance to health, as it serves as a vector of several pathogens, including  Theileria orientalis ,  Babesia ovata ,  Rickettsia japonica  and the severe fever with thrombocytopenia syndrome virus (SFTSV). Presently, the major method of control for this tick is the use of chemical acaricides. The glutathione S-transferase (GST) system is one mechanism through which the tick metabolizes these acaricides. Two GSTs from  H. longicornis  (HlGST and HlGST2) have been previously identified.    Results  Enzyme kinetic studies were performed to determine the interaction of acaricides with recombinant  H. longicornis  GSTs. Recombinant HlGST activity was inhibited by flumethrin and cypermethrin, while recombinant HlGST2 activity was inhibited by chlorpyrifos and cypermethrin. Using real-time RT-PCR, the upregulation of the  HlGST  gene was observed upon exposure to sublethal doses of flumethrin, while the  HlGST2  gene was upregulated when exposed to sublethal doses of chlorpyrifos. Sex and strain dependencies in the induction of  GST  gene expression by flumethrin were also observed. Knockdown of the  HlGST  gene resulted in the increased susceptibility of larvae and adult male ticks to sublethal doses of flumethrin and the susceptibility of larvae against sublethal doses of chlorpyrifos was increased upon knockdown of  HlGST2 .    Conclusions  HlGST could be vital for the metabolism of flumethrin in larvae and adult male ticks, while HlGST2 is important in the detoxification of chlorpyrifos in larval ticks.    Electronic supplementary material  The online version of this article (10.1186/s13071-018-3044-9) contains supplementary material, which is available to authorized users.

Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis