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Background  The strongylid parasite  Haemonchus contortus  causes severe anemia in domestic animals worldwide. Effective preventive and therapeutical agents are lacking, because of drug resistance and that little is known about the molecular mechanism of the interaction between  H. contortus  and host cells.    Methods  A new gene,  Hc-clec-160 , was discovered with RT-PCR. Transcriptional levels of  Hc-clec-160  and  Ce-clec-160  throughout different growth phases of corresponding nematodes were assayed by qPCR. Immunofluorescence staining of paraffin section were performed to determine the protein localization in adult worms of  H. contortus . To monitor the promoter capacity of the 5'-flanking region of  Ce-clec-160 , micro-injection was used. Overexpression and RNAi constructs was carried out in the N2 strain of  Caenorhabditis elegans  to find out the gene function of  Hc-clec-160 .    Results  The full-length cDNA of 1224 bp of  Hc-clec-160  was cloned by RT-PCR. The corresponding gene contained twelve exons. Its transcripts peaked in male adult worms. Hc-CLEC-160 was predicted to have a Willebrand factor type A (vWA) domain and a C-type lectin domain. The proteins were not detected by expression in  C. elegans  or paraffin section experiments in adult of  H. contortus . Knockdown of  Ce-clec-160  expression in  C. elegans  by RNAi resulted in shortened body length and decreased brood size.    Conclusions  In this experiment, a new gene  Hc-clec-160  was obtained in  H. contortus  and its function was addressed using a model organism:  C. elegans . Our study showed that  Hc-clec-160  possesses characteristics similar to those of  Ce-clec-160  and plays an important role in the growth and reproduction of this parasitic nematode.    Electronic supplementary material  The online version of this article (10.1186/s13071-018-3005-3) contains supplementary material, which is available to authorized users.

parasites and vectors

Identification and preliminary characterization of Hc-clec-160, a novel C-type lectin domain-containing gene of the strongylid nematode Haemonchus contortus