English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   Melophagus ovinus  (sheep ked) is a blood-feeding ectoparasite that belongs to the family Hippoboscidae (Diptera: Hippoboscoidea) and mainly parasitizes sheep. The life-cycle of  M. ovinus  consists of three stages: larva, pupa and adult. It has a worldwide distribution and has been found in four provinces of China, especially South Xinjiang. In addition to causing direct damage to animal hosts,  M. ovinus  serves as a vector for disease transmission. In this study, our aim was to investigate the presence of  Anaplasma  spp. in pupal and adult  M. ovinus .    Methods  A total of 93 specimens (including eight pupal specimens) of  M. ovinus  collected in South Xinjiang were selected for isolation of genomic DNA, followed by PCR amplification and sequencing of the  msp4  gene of  Anaplasma  spp. The sequences were analyzed in MEGA 7.0 software and  via  online BLAST.    Results  PCR and sequencing results showed that all the specimens collected in 2013 were free of  Anaplasma  spp., whereas three and 25 specimens (including five pupal specimens) collected in 2016 and 2017, respectively, tested positive for  Anaplasma  spp. The analysis of 24  msp4  gene sequences (from four pupal specimens) confirmed the presence of  A. ovis  in  M. ovinus  specimens collected in South Xinjiang, China. The detected  A. ovis  isolates belong to Genotypes II and III.    Conclusions  To the best of our knowledge, this is the first report of the detection of  A. ovis  DNA in pupal  M. ovinus , confirming the vertical transmission of  A. ovis  in  M. ovinus  and the potential of  M. ovinus  to serve as a vector for  A. ovis .

First report of Anaplasma ovis in pupal and adult Melophagus ovinus (sheep ked) collected in South Xinjiang, China