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Background  Mass egg production is an important component of  Aedes albopictus  mosquito control programs, such as the sterile insect technique and incompatible insect technique, which requires the releases of large number of sterile males. Developing standard operating procedures and optimized cages for adult maintenance of  Ae. albopictus  can improve the mass rearing efficiency.    Methods  Three different sex ratios of females to males with a total number of 4,000 mosquitoes were tested by evaluating the insemination rate, egg production (total number of eggs per cage), female fecundity and egg hatch rate in small cage (30 × 30 × 30 cm). Blood meals with adenosine triphosphate (ATP, 0.05 g/ml), cage structures (Big cage A: 90 × 30 × 30 cm; Big cage B: 90 × 30 × 50 cm or 90 × 50 × 30 cm) and rearing densities (12,000, 16,000 and 20,000 mosquitoes, corresponding to 0.9 cm 2 /mosquito, 0.675 cm 2 /mosquito and 0.54 cm 2 /mosquito, respectively) were also tested and evaluated on the basis of egg production, female fecundity and egg hatch rate. An adult rearing unit holding 15 of Big cage A with optimal egg production was designed to produce 10 million eggs per rearing cycle in a 1.8 m 2  space.    Results  Female to male ratios at 3:1 in small cages resulted in higher egg production but did not affect insemination rate, female fecundity and egg hatch rate. A concentration of 0.05 g/ml of ATP added to blood meals improved the blood-feeding frequency and thus increased the overall egg production per cage. Cage structures affected the egg production per cage, but not egg hatch rate. A medium rearing density at 0.675 cm 2 /mosquito (16,000 mosquitoes) resulted in higher egg production compared to both low and high densities. An adult rearing unit for  Ae. albopictus  on the basis of Big cage A has been developed with the capacity of producing 10 million eggs within 15 days.    Conclusions  Our results have indicated that the adult rearing methods and adult maintenance unit are recommended for  Ae. albopictus  mass rearing in support of the establishment of a medium-sized mosquito factory.    Electronic supplementary material  The online version of this article (10.1186/s13071-018-2750-7) contains supplementary material, which is available to authorized users.

Establishment of a medium-scale mosquito facility: tests on mass production cages for Aedes albopictus (Diptera: Culicidae)