English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Background  Despite the abundance of the domestic cat flea,  Ctenocephalides felis  (Bouché, 1835) and disease risks associated with them, flea control is difficult and requires the development of new control interventions such as vaccines. In this study, a reverse vaccinology approach was designed to achieve a rational selection of cat flea candidate protective antigens.    Methods  Based on transcriptomics and proteomics data from unfed adult fleas it was possible to select more specific candidate protective antigens based on highly represented and functionally relevant proteins present in the predicted exoproteome. The protective capacity of the recombinant antigens was evaluated for the control of  C. felis  infestations in vaccinated cats.    Results  Vaccination with recombinant antigens induced an antibody response in immunized cats. Furthermore, a correlation was obtained between the effect of vaccination (antibody levels) and vaccine efficacy on flea phenotype (egg hatchability). The results suggested that the main effect of vaccination with these antigens was on reducing cat flea egg hatchability and fertility, with an overall vaccine efficacy of 32–46%. Although vaccination with these antigens did not have an effect on flea infestations, vaccines affecting reproductive capacity could reduce cat flea populations, particularly under conditions of direct insect transmission between cats.    Conclusions  These results support the development of vaccines with protective antigens affecting flea reproduction and development after feeding on immunized animals for the control of cat flea infestations.    Electronic supplementary material  The online version of this article (10.1186/s13071-018-2618-x) contains supplementary material, which is available to authorized users.

parasites and vectors

A reverse vaccinology approach to the identification and characterization of Ctenocephalides felis candidate protective antigens for the control of cat flea infestations