English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

ᅟ  Previous reports suggest that the 2-methyl butyramide and 2-methyl valeramide metabolites of  Ascaris lumbricoides  in urine of infected individuals could be considered as urinary biomarkers for active infection. We have developed an LC-MS method with a detection limit of 10 ng/mL using synthetic chemicals as reference material. Urine samples ( n  = 21) of infected individuals were analyzed for the presence of these metabolites, but they were not detected in any of the samples. Furthermore, the recorded  1 H-NMR spectrum for reference 2-methyl butyramide did not match with the spectrum that was described for the  Ascaris  metabolite. Based on these two observations, we concluded that the urinary biomarkers that were detected for  A. lumbricoides  infection are not 2-methyl butyramide nor 2-methylvaleramide. New discovery efforts will be required to identify the structure of these metabolite biomarkers in urine of infected individuals.    Trial registration  Urine samples used in this study were collected as part of a clinical trial with trial number  ISRCTN75636394  (12 November 2013).    Electronic supplementary material  The online version of this article (10.1186/s13071-017-2600-z) contains supplementary material, which is available to authorized users.

2-methyl butyramide, a previously identified urine biomarker for Ascaris lumbricoides, is not present in infected Indonesian individuals